| 1. | Collection and preservation of first polar body of oocyte in mice
小鼠卵母细胞第一极体的采集与保存 |
| 2. | The effect of icsi in the site of the first polar body of oocyte
第一极体位置对卵胞浆内单精子显微注射结局的影响 |
| 3. | The first polar body of human oocytes does not help predict the precise location of the spindle
第一极体不能精确定位人卵母细胞纺锤体 |
| 4. | First polar body
第一极体 |
| 5. | The influence of the first polar body position at intracytoplasmic sperm injection on fertilization and embryo development
第一极体位置对卵母细胞显微注射受精后受精和胚胎发育的影响 |
| 6. | Effects of the first polar body ' s position and morphology in murine oocyte on fertility rate and fertilized egg quality in intracytoplasmic sperm injection
卵胞浆内单精子注射中小鼠卵细胞第一极体位置和形态对受精率和受精卵质量的影响 |
| 7. | After 15h of maturation . 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended . secondly , strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium
第一次减数分裂期小鼠卵母细胞经化学去核后，去除透明带，并胎儿成纤维细胞粘合、电融合并srcl2激活，培养于微孔中。 |
| 8. | These results indicate that chx at an app ropriate concentration can improve the parthenogenetic development of buffalo oocytes , cb at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei
结果表明，适当浓度的chx能提高卵母细胞激活效果，适当浓度的cb可抑制卵母细胞第二极体的排出，提高孤雌激活后双原核的比例。 2 |
| 9. | The second medium included egf ing / ml and igf - 1 long / ml in addition to the control . likewise , the last medium contained 50 % granulosa cells conditioned medium . oocytes were cultured for 16 or 18 hours , and only the oocytes underwent first polar body extrusion were collected for future study
Inmegflug ml十igf 110ng ml ）和颗粒细胞生长液组乃0颗粒细胞生长液） ，对昆明白小鼠生发泡期（ ge讪nalvesicle ， gv ）卵母细胞进行体外成熟培养； 5 |
| 10. | Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。 |
